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stat5 inhibition  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology stat5 inhibition
    FIGURE 4 lTSLP induces pro-inflammatory cytokine expression in PBMCs by triggering <t>STAT5</t> activation. (A-D) PBMCs from RA patients and healthy controls (HC) were cultured for 24h with or without LPS (100 ng/mL) or lTSLP (100 ng/mL). Relative mRNA levels of Il-1b (A), Il-6 (B), Il-8 (C) and Il-10 (D) were analyzed by RT-qPCR. (E) PBMCs from RA patients were treated 24h with 0.25 µg/ml anti-TSLP neutralizing antibody or an IgG antibody control, and the relative mRNA levels of Il-1b, Il-6, Il-8, and Il-10 were analyzed by RT-qPCR. (F) PBMCs from HC and RA were stimulated with 100 ng/mL LPS in the presence or absence of 100 ng/mL lTSLP for 1h. Western blots to examine the expression level of p-STAT5, normalized to b-actin. Relative p-STAT5 expression was quantified in (G). (H) PBMCs from RA patients were treated with 50 mM STAT5 inhibitor 1h before being stimulated with LPS (100 ng/mL) and lTSLP (100 ng/mL) for RT-PCR detection of Il-1b, Il-6, Il-8, and Il-10 mRNA expression. P values were measured by unpaired two-tailed Student’s t- test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significant. Error bar represent standard deviation.
    Stat5 Inhibition, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stat5 inhibition/product/Santa Cruz Biotechnology
    Average 94 stars, based on 34 article reviews
    stat5 inhibition - by Bioz Stars, 2026-03
    94/100 stars

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    1) Product Images from "Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses."

    Article Title: Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses.

    Journal: Frontiers in immunology

    doi: 10.3389/fimmu.2022.1079415

    FIGURE 4 lTSLP induces pro-inflammatory cytokine expression in PBMCs by triggering STAT5 activation. (A-D) PBMCs from RA patients and healthy controls (HC) were cultured for 24h with or without LPS (100 ng/mL) or lTSLP (100 ng/mL). Relative mRNA levels of Il-1b (A), Il-6 (B), Il-8 (C) and Il-10 (D) were analyzed by RT-qPCR. (E) PBMCs from RA patients were treated 24h with 0.25 µg/ml anti-TSLP neutralizing antibody or an IgG antibody control, and the relative mRNA levels of Il-1b, Il-6, Il-8, and Il-10 were analyzed by RT-qPCR. (F) PBMCs from HC and RA were stimulated with 100 ng/mL LPS in the presence or absence of 100 ng/mL lTSLP for 1h. Western blots to examine the expression level of p-STAT5, normalized to b-actin. Relative p-STAT5 expression was quantified in (G). (H) PBMCs from RA patients were treated with 50 mM STAT5 inhibitor 1h before being stimulated with LPS (100 ng/mL) and lTSLP (100 ng/mL) for RT-PCR detection of Il-1b, Il-6, Il-8, and Il-10 mRNA expression. P values were measured by unpaired two-tailed Student’s t- test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significant. Error bar represent standard deviation.
    Figure Legend Snippet: FIGURE 4 lTSLP induces pro-inflammatory cytokine expression in PBMCs by triggering STAT5 activation. (A-D) PBMCs from RA patients and healthy controls (HC) were cultured for 24h with or without LPS (100 ng/mL) or lTSLP (100 ng/mL). Relative mRNA levels of Il-1b (A), Il-6 (B), Il-8 (C) and Il-10 (D) were analyzed by RT-qPCR. (E) PBMCs from RA patients were treated 24h with 0.25 µg/ml anti-TSLP neutralizing antibody or an IgG antibody control, and the relative mRNA levels of Il-1b, Il-6, Il-8, and Il-10 were analyzed by RT-qPCR. (F) PBMCs from HC and RA were stimulated with 100 ng/mL LPS in the presence or absence of 100 ng/mL lTSLP for 1h. Western blots to examine the expression level of p-STAT5, normalized to b-actin. Relative p-STAT5 expression was quantified in (G). (H) PBMCs from RA patients were treated with 50 mM STAT5 inhibitor 1h before being stimulated with LPS (100 ng/mL) and lTSLP (100 ng/mL) for RT-PCR detection of Il-1b, Il-6, Il-8, and Il-10 mRNA expression. P values were measured by unpaired two-tailed Student’s t- test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significant. Error bar represent standard deviation.

    Techniques Used: Expressing, Activation Assay, Cell Culture, Quantitative RT-PCR, Control, Western Blot, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test, Standard Deviation



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    FIGURE 4 lTSLP induces pro-inflammatory cytokine expression in PBMCs by triggering <t>STAT5</t> activation. (A-D) PBMCs from RA patients and healthy controls (HC) were cultured for 24h with or without LPS (100 ng/mL) or lTSLP (100 ng/mL). Relative mRNA levels of Il-1b (A), Il-6 (B), Il-8 (C) and Il-10 (D) were analyzed by RT-qPCR. (E) PBMCs from RA patients were treated 24h with 0.25 µg/ml anti-TSLP neutralizing antibody or an IgG antibody control, and the relative mRNA levels of Il-1b, Il-6, Il-8, and Il-10 were analyzed by RT-qPCR. (F) PBMCs from HC and RA were stimulated with 100 ng/mL LPS in the presence or absence of 100 ng/mL lTSLP for 1h. Western blots to examine the expression level of p-STAT5, normalized to b-actin. Relative p-STAT5 expression was quantified in (G). (H) PBMCs from RA patients were treated with 50 mM STAT5 inhibitor 1h before being stimulated with LPS (100 ng/mL) and lTSLP (100 ng/mL) for RT-PCR detection of Il-1b, Il-6, Il-8, and Il-10 mRNA expression. P values were measured by unpaired two-tailed Student’s t- test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significant. Error bar represent standard deviation.
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    FIGURE 4 lTSLP induces pro-inflammatory cytokine expression in PBMCs by triggering <t>STAT5</t> activation. (A-D) PBMCs from RA patients and healthy controls (HC) were cultured for 24h with or without LPS (100 ng/mL) or lTSLP (100 ng/mL). Relative mRNA levels of Il-1b (A), Il-6 (B), Il-8 (C) and Il-10 (D) were analyzed by RT-qPCR. (E) PBMCs from RA patients were treated 24h with 0.25 µg/ml anti-TSLP neutralizing antibody or an IgG antibody control, and the relative mRNA levels of Il-1b, Il-6, Il-8, and Il-10 were analyzed by RT-qPCR. (F) PBMCs from HC and RA were stimulated with 100 ng/mL LPS in the presence or absence of 100 ng/mL lTSLP for 1h. Western blots to examine the expression level of p-STAT5, normalized to b-actin. Relative p-STAT5 expression was quantified in (G). (H) PBMCs from RA patients were treated with 50 mM STAT5 inhibitor 1h before being stimulated with LPS (100 ng/mL) and lTSLP (100 ng/mL) for RT-PCR detection of Il-1b, Il-6, Il-8, and Il-10 mRNA expression. P values were measured by unpaired two-tailed Student’s t- test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significant. Error bar represent standard deviation.
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    FIGURE 4 lTSLP induces pro-inflammatory cytokine expression in PBMCs by triggering STAT5 activation. (A-D) PBMCs from RA patients and healthy controls (HC) were cultured for 24h with or without LPS (100 ng/mL) or lTSLP (100 ng/mL). Relative mRNA levels of Il-1b (A), Il-6 (B), Il-8 (C) and Il-10 (D) were analyzed by RT-qPCR. (E) PBMCs from RA patients were treated 24h with 0.25 µg/ml anti-TSLP neutralizing antibody or an IgG antibody control, and the relative mRNA levels of Il-1b, Il-6, Il-8, and Il-10 were analyzed by RT-qPCR. (F) PBMCs from HC and RA were stimulated with 100 ng/mL LPS in the presence or absence of 100 ng/mL lTSLP for 1h. Western blots to examine the expression level of p-STAT5, normalized to b-actin. Relative p-STAT5 expression was quantified in (G). (H) PBMCs from RA patients were treated with 50 mM STAT5 inhibitor 1h before being stimulated with LPS (100 ng/mL) and lTSLP (100 ng/mL) for RT-PCR detection of Il-1b, Il-6, Il-8, and Il-10 mRNA expression. P values were measured by unpaired two-tailed Student’s t- test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significant. Error bar represent standard deviation.

    Journal: Frontiers in immunology

    Article Title: Increased expression of long-isoform thymic stromal lymphopoietin is associated with rheumatoid arthritis and fosters inflammatory responses.

    doi: 10.3389/fimmu.2022.1079415

    Figure Lengend Snippet: FIGURE 4 lTSLP induces pro-inflammatory cytokine expression in PBMCs by triggering STAT5 activation. (A-D) PBMCs from RA patients and healthy controls (HC) were cultured for 24h with or without LPS (100 ng/mL) or lTSLP (100 ng/mL). Relative mRNA levels of Il-1b (A), Il-6 (B), Il-8 (C) and Il-10 (D) were analyzed by RT-qPCR. (E) PBMCs from RA patients were treated 24h with 0.25 µg/ml anti-TSLP neutralizing antibody or an IgG antibody control, and the relative mRNA levels of Il-1b, Il-6, Il-8, and Il-10 were analyzed by RT-qPCR. (F) PBMCs from HC and RA were stimulated with 100 ng/mL LPS in the presence or absence of 100 ng/mL lTSLP for 1h. Western blots to examine the expression level of p-STAT5, normalized to b-actin. Relative p-STAT5 expression was quantified in (G). (H) PBMCs from RA patients were treated with 50 mM STAT5 inhibitor 1h before being stimulated with LPS (100 ng/mL) and lTSLP (100 ng/mL) for RT-PCR detection of Il-1b, Il-6, Il-8, and Il-10 mRNA expression. P values were measured by unpaired two-tailed Student’s t- test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, no significant. Error bar represent standard deviation.

    Article Snippet: For STAT5 inhibition, PBMCs from RA patients were treated with or without 50 mM of STAT5 inhibitor (Santa Cruz, sc-355979) for 1h before LPS and lTSLP stimulation for 24h.

    Techniques: Expressing, Activation Assay, Cell Culture, Quantitative RT-PCR, Control, Western Blot, Reverse Transcription Polymerase Chain Reaction, Two Tailed Test, Standard Deviation